How do tlc work

Works well for all compounds that can be oxidized. Yellow on purple. Yellow or light brown on purple. Bromocresol Green. Yellow spot on blue background. Good general stain, very well with polyhydroxylated and carbonyl compounds.

Blue or green spot. Upon heating, very sensitive! Good general stain, particularly sensitive towards nucleophiles. Varying colors on light pink plate upon heating. Does not work with alkenes, alkynes or aromatic system unless functional groups are present. Phosphomolybdic acid PMA. Very sensitive. Dark green spot on light green plate. Sensitivity can be enhanced by use of cobalt II chloride.

Indoles, amines. Pink or red-violet. Dragendorff-Munier Stain. Amines even the ones that are low in reactivity. Various colors. Due to the fact that all those variables are difficult to keep constant, a reference compound is usually applied to the plate as well. TLC University of Colorado. TLC Macherey Nagel. TLC chamber for development e. Yellow on purple Yellow or light brown on purple.

Its widespread use can be attributed to both its inertness and its porous structure. The silica gel in TLC is mounted on the slide, or solid support. A number of different solvents can be employed as mobile phases for a TLC experiment. Because a mobile phase must be able to transport an analyte, inorganic solvents only work with inorganic materials, and organic solvents can work with either organic or inorganic materials.

Confinement of a TLC plate in a chamber which has its headspace the air in the chamber saturated with solvent vapor allows for elution of a sample by capillary action. The solvent simply rises up the slide and brings the analyte with it. Solvents are not strictly one and only one compound. Different solvents can be mixed in varying ratios to achieve a good separation. Preparation Prepare the TLC chamber, which can be as simple as a mason jar, with the solvent system. Close the lid to the TLC chamber and allow to equilibrate for about 10 minutes or more.

Turn on hot plate to a low setting. Prepare visualization chamber by placing some resublimed iodine crystals into a mason jar and secure the lid. Procedure Using 2 small pieces of tape, attach the TLC plate to the hotplate such that the bottom of the plate is touching the hotplate. Mark the origin bottom of your plate with a pencil and label each lane. Secure the lid to the chamber and allow the plate to develop. Once the solvent front nears the top of the plate, remove the plate from the TLC chamber, quickly mark the solvent front with a pencil, and allow it to dry.

It typically takes minutes for the solvent front to reach near the top of the plate. Place the plate into the iodine chamber for visualization and secure the lid. What conclusions can you draw about the polarity of the substances?

Discuss one purpose of this technique in research or industry. Place the plate in charged TLC chamber, in front of the wick. Secure lid to chamber and allow the pl….

TLC plate progression. High School. Science Saturday. Learning Resources.